Rapid Viral Vector Characterization
The KaritroMP makes it easy to quickly characterize samples of large viral vectors and viral-like particles (VLPs). Leveraging innovative macro mass photometry technology, the KaritroMP’s unique functionality gives it an edge in terms of speed, ease of use and the information it provides.
Through single-particle analysis, the KaritroMP enables users to visualize the distributions of particles present in their samples in minutes. Measuring two distinct parameters for every particle, it resolves distinct populations provides enhanced resolution compared to traditional techniques like DLS/NTA.
Just as Refeyn’s SamuxMP mass photometer returns high-performance analytical solutions AAV analytics in just minutes and the TwoMP mass photometer offers fast and effective RNA analytics, the KaritroMP quickly returns valuable new insights into the purity, stability and composition of large viral vector samples. The Karitro joins the Samux and TwoMP to create a powerful analytical toolbox for cell and gene therapy, and vaccine development – for every modality.
Key Features
1. Characterization of large viral vectors and VLPs
2. Semi-automated workflow
3. Fast and easy to use
For each particle in a sample, the KaritroMP measures two distinct parameters: Contrast (a qualitative measure related to the particle’s composition and mass) and size.
Resolution of empty/full ratios
Because it measures contrast and mass, the instrument can distinguish populations of particles with the same size but different mass. For example, it can resolve empty vs. full adenovirus capsids and quantify their proportions (Fig. 1). Comparing data from the KaritroMP with dynamic light scattering (DLS) and nanoparticle tracking analysis (NTA) shows that only the Karitro could resolve the empty and full populations. With both NTA and DLS, only one population was apparent.
Its speed and excellent contrast resolution make the KaritroMPone of the few instruments currently available that can quickly and directly measure AdV empty-full ratios.
Figure 1. The KaritroMP resolves empty/full AdV populations that cannot be resolved using NTA or DLS. Full and empty populations were clearly resolved via KaritroMP analysis of a mixture containing empty, fragmented and full AdV capsids.
（Please refer to the product mannual for information details.）
